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KMID : 1094720140190061034
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 6 p.1034 ~ p.1041
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Biodegradation of 4-aminobenzenesulfonate by indigenous isolate Shinella yambaruensis SA1 and its validation by genotoxic analysis
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Biala Sunil
Chadha Pooja
Saini Harvinder Singh
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Abstract
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Shinella yambaruensis SA1, an indigenous strain, was isolated from sludge of wastewater treatment plant. The isolate SA1 efficiently degraded 0.58 mM of 4-aminobenzenesulfonate (4-ABS) after 6 h incubation and utilized it as a sole source of carbon, nitrogen and energy under aerobic conditions. The isolate SA1 retained its potential to degrade 4-ABS when grown in medium supplemented upto 3% (w/v) NaCl. The LC/MS based analysis of degradation products and assay of ring opening enzymes indicated that 4-ABS was degraded via ortho cleavage pathway. The efficiency of 4-ABS biodegradation was evaluated by scoring chromosomal aberrations in mice which showed 53% reduction in the genotoxicity of biologically treated samples as compared to pure 4-ABS. Thus, SA1 could be a potential isolate for developing sequential anoxic-aerobic system for achieving mineralization of polar aromatic amine intermediates generated after breakdown of textile dyes.
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KEYWORD
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4-aminobenzenesulfonate, biodegradation, genotoxicity, Shinella yambaruensis, textile industry effluent
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